9KQ6
The structure of the apo-YcfA from Erwinia amylovora
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-08-26 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97853 |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 69.758, 134.923, 234.270 |
| Unit cell angles | 90.00, 98.55, 90.00 |
Refinement procedure
| Resolution | 48.280 - 2.340 |
| R-factor | 0.24847 |
| Rwork | 0.247 |
| R-free | 0.28470 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 2.066 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0430) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.280 | 2.406 |
| High resolution limit [Å] | 2.340 | 2.345 |
| Number of reflections | 178211 | 16975 |
| <I/σ(I)> | 10.97 | |
| Completeness [%] | 99.3 | |
| Redundancy | 4.1 | |
| CC(1/2) | 0.996 | 0.463 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289.15 | 3.5% Tacsimate and 25% PEG3350 |
