9KQ4

The structure of YcfC from Erwinia amylovora as a C-S lyase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-08-26
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979
Spacegroup name	P 43 21 2
Unit cell lengths	152.619, 152.619, 73.331
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.800 - 2.090
Rwork	0.185
R-free	0.22990
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.017
RMSD bond angle	2.469
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.800	2.142
High resolution limit [Å]	2.090	2.090
Rpim	0.049
Number of reflections	50350	4237
<I/σ(I)>	14.67
Completeness [%]	93.4
Redundancy	20
CC(1/2)		0.844

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289.15	50 mM sodium formate