9KPW

UDP-glycosyltransferase TsUGT1 with UDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-05-29
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.979
Spacegroup name	P 1
Unit cell lengths	50.746, 89.258, 113.182
Unit cell angles	93.74, 97.61, 104.31

Refinement procedure

Resolution	28.680 - 2.750
R-factor	0.1932
Rwork	0.190
R-free	0.24720
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.471
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.15.1_3469)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.680	2.848
High resolution limit [Å]	2.750	2.750
Rmerge	0.076
Number of reflections	88146	9584
<I/σ(I)>	6.82
Completeness [%]	91.2	97.34
Redundancy	2
CC(1/2)	0.984	0.861

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	291.15	20mM Tris 100mM NaCl, 5mM DTT