9KPU
Crystal structure of FrazP2 in complex with forazoline C
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL10U2 |
| Synchrotron site | SSRF |
| Beamline | BL10U2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-05-01 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9785 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.120, 74.110, 103.190 |
| Unit cell angles | 90.00, 98.13, 90.00 |
Refinement procedure
| Resolution | 43.420 - 2.300 |
| R-factor | 0.21812 |
| Rwork | 0.215 |
| R-free | 0.27612 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.785 |
| Data scaling software | Aimless |
| Phasing software | PHASES |
| Refinement software | REFMAC (5.8.0430) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.420 | 2.382 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 33285 | 3579 |
| <I/σ(I)> | 22.45 | |
| Completeness [%] | 96.8 | |
| Redundancy | 6.5 | |
| CC(1/2) | 0.999 | 0.997 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.085 M Sodium acetate trihydrate (pH 4.6), 25.5 % PEG 4000, 0.17 M Ammonium acetate and 15 % Glycerol |
