9KPL
Crystal structure of T. rubripes Mincle with glucose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-1A |
| Synchrotron site | Photon Factory |
| Beamline | BL-1A |
| Temperature [K] | 95 |
| Detector technology | PIXEL |
| Collection date | 2024-02-18 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 1.0200 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.353, 69.860, 51.584 |
| Unit cell angles | 90.00, 115.77, 90.00 |
Refinement procedure
| Resolution | 46.450 - 1.800 |
| R-factor | 0.1816 |
| Rwork | 0.180 |
| R-free | 0.21550 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.533 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.21_5207) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.450 | 1.840 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.066 | 0.369 |
| Rpim | 0.041 | 0.226 |
| Number of reflections | 28652 | 1716 |
| <I/σ(I)> | 8.6 | |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 3.5 | 3.6 |
| CC(1/2) | 0.998 | 0.874 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.1M tris (pH8.5), 0.2M sodium chloride, 25% (w/v) polyethylene glycol (PEG) 3350 |
