9KPL
Crystal structure of T. rubripes Mincle with glucose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-1A |
Synchrotron site | Photon Factory |
Beamline | BL-1A |
Temperature [K] | 95 |
Detector technology | PIXEL |
Collection date | 2024-02-18 |
Detector | DECTRIS EIGER X 4M |
Wavelength(s) | 1.0200 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 48.353, 69.860, 51.584 |
Unit cell angles | 90.00, 115.77, 90.00 |
Refinement procedure
Resolution | 46.450 - 1.800 |
R-factor | 0.1816 |
Rwork | 0.180 |
R-free | 0.21550 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.019 |
RMSD bond angle | 1.533 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | PHENIX (1.21_5207) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.450 | 1.840 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.066 | 0.369 |
Rpim | 0.041 | 0.226 |
Number of reflections | 28652 | 1716 |
<I/σ(I)> | 8.6 | |
Completeness [%] | 99.7 | 100 |
Redundancy | 3.5 | 3.6 |
CC(1/2) | 0.998 | 0.874 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.1M tris (pH8.5), 0.2M sodium chloride, 25% (w/v) polyethylene glycol (PEG) 3350 |