9KPB
Crystal structure of human CASTOR2 in apo form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-11-21 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97853 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 105.516, 79.796, 62.746 |
| Unit cell angles | 90.00, 120.00, 90.00 |
Refinement procedure
| Resolution | 32.120 - 2.500 |
| R-factor | 0.1918 |
| Rwork | 0.189 |
| R-free | 0.24130 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5gs9 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.943 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.140 | 2.590 |
| High resolution limit [Å] | 2.490 | 2.490 |
| Rmerge | 0.081 | 0.762 |
| Number of reflections | 12429 | 580 |
| <I/σ(I)> | 12.4 | 2.1 |
| Completeness [%] | 79.0 | 39.4 |
| Redundancy | 5 | 4.7 |
| CC(1/2) | 0.998 | 0.755 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 1 M Succinic acid, pH 7.0, and 15% PEG 3350 |
