9KPA
Crystal structure of Horse spleen L-ferritin mutant (R59F)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E DW |
| Temperature [K] | 93 |
| Detector technology | PIXEL |
| Collection date | 2021-04-19 |
| Detector | RIGAKU HyPix-6000HE |
| Wavelength(s) | 1.54184 |
| Spacegroup name | F 4 3 2 |
| Unit cell lengths | 180.843, 180.843, 180.843 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 18.460 - 1.500 |
| R-factor | 0.1806 |
| Rwork | 0.180 |
| R-free | 0.19440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dat |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.737 |
| Data reduction software | CrysalisPro |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 18.460 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.053 | 0.388 |
| Rmeas | 0.056 | 0.421 |
| Rpim | 0.017 | 0.161 |
| Number of reflections | 40986 | 2014 |
| <I/σ(I)> | 30.8 | 4.7 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 10.1 | 6.7 |
| CC(1/2) | 1.000 | 0.938 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | Ammonium sulfate, Cadmium sulfate |
