Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-1A |
| Synchrotron site | Photon Factory |
| Beamline | BL-1A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-10-30 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 1.012 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 92.498, 92.498, 105.120 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 80.110 - 2.130 |
| R-factor | 0.16841 |
| Rwork | 0.166 |
| R-free | 0.20557 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.684 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 80.110 | 2.170 |
| High resolution limit [Å] | 2.130 | 2.130 |
| Rmerge | 0.146 | 0.982 |
| Rmeas | 0.159 | 1.077 |
| Rpim | 0.060 | 0.429 |
| Number of reflections | 29428 | 1440 |
| <I/σ(I)> | 7.8 | 1.7 |
| Completeness [%] | 99.5 | |
| Redundancy | 6.7 | |
| CC(1/2) | 0.993 | 0.527 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.08 M MES-NaOH pH 6.0, 16%(w/v) PEG6000, and 0.16 M NaCl |
