9KP1

Heterodimer of heptaprenyl diphosphate synthase from B. subtilis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-03-01
Detector	DECTRIS EIGER X 4M
Wavelength(s)	0.97931
Spacegroup name	C 2 2 21
Unit cell lengths	84.865, 145.199, 124.736
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.492 - 2.484
R-factor	0.2177
Rwork	0.215
R-free	0.26000
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.849
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.500	2.520
High resolution limit [Å]	2.484	2.484
Rmerge	0.077	0.447
Number of reflections	27421	1340
<I/σ(I)>	38.2	8
Completeness [%]	100.0
Redundancy	13.2
CC(1/2)	1.000	0.965

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.05M Ammonium sulfate, 0.05M Bis-Tris, pH 6.5, 30% (15/4) EO/OH