9KP0
Crystal structure of Oryza sativa HPPD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-1A |
| Synchrotron site | Photon Factory |
| Beamline | BL-1A |
| Temperature [K] | 95 |
| Detector technology | PIXEL |
| Collection date | 2024-03-19 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 1.02 |
| Spacegroup name | P 1 |
| Unit cell lengths | 59.110, 64.590, 73.160 |
| Unit cell angles | 101.39, 107.96, 97.00 |
Refinement procedure
| Resolution | 45.940 - 2.000 |
| Rwork | 0.194 |
| R-free | 0.22650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1sp9 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.315 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0430) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.120 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.168 | 0.842 |
| Number of reflections | 65758 | 10263 |
| <I/σ(I)> | 6.92 | 2.03 |
| Completeness [%] | 97.9 | 95.3 |
| Redundancy | 3.69 | 3.32 |
| CC(1/2) | 0.978 | 0.622 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M sodium acetate (pH 4.5), 20 vol% PEG1000, and 0.2 M zinc acetate 2 mM CoCl2 |
