9KOY
Crystal structure of Oryza sativa HPPD complexed with iptriazopyrid
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-1A |
| Synchrotron site | Photon Factory |
| Beamline | BL-1A |
| Temperature [K] | 95 |
| Detector technology | PIXEL |
| Collection date | 2024-05-22 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 1.018 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 158.610, 158.610, 84.075 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.652 - 2.900 |
| Rwork | 0.203 |
| R-free | 0.28550 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1sp9 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.457 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.060 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.260 | 3.084 |
| Number of reflections | 24533 | 3836 |
| <I/σ(I)> | 12.39 | 0.89 |
| Completeness [%] | 99.8 | 99.3 |
| Redundancy | 13.4 | 13.9 |
| CC(1/2) | 0.997 | 0.379 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M sodium citrate (pH 5.0), 12 weight% PEG6000, and 50 mM sodium iodide 2 mM iptriazopyrid and 2 mM CoCl2 |
