9KOV
Crystal structure of an amidohydrolase mutant from Thermonema
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL10U2 |
| Synchrotron site | SSRF |
| Beamline | BL10U2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-10-26 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.979183 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 84.645, 205.755, 105.268 |
| Unit cell angles | 90.00, 103.08, 90.00 |
Refinement procedure
| Resolution | 49.920 - 2.560 |
| R-factor | 0.2241 |
| Rwork | 0.222 |
| R-free | 0.26350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.239 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.21_5207: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.710 |
| High resolution limit [Å] | 2.560 | 2.560 |
| Number of reflections | 112801 | 18192 |
| <I/σ(I)> | 6.86 | 2.07 |
| Completeness [%] | 98.6 | 99.8 |
| Redundancy | 7.9 | 6.7 |
| CC(1/2) | 0.983 | 0.645 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.1M sodium acetate, 2.0M ammonium sulfate |
