9KO7
Crystal structure of chicken ACE2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-09-13 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.98 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 169.983, 92.781, 152.709 |
| Unit cell angles | 90.00, 91.82, 90.00 |
Refinement procedure
| Resolution | 44.270 - 3.270 |
| R-factor | 0.2425 |
| Rwork | 0.239 |
| R-free | 0.29590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.341 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.19.1_4122: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.380 |
| High resolution limit [Å] | 3.270 | 3.270 |
| Number of reflections | 36661 | 3585 |
| <I/σ(I)> | 7.6 | |
| Completeness [%] | 98.4 | |
| Redundancy | 6.5 | |
| CC(1/2) | 0.934 | 0.882 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291.15 | 0.2 M Ammonium sulfate 0.1 M BIS-TRIS pH5.5 25% Polyethylene glycol 3350 |
