9KNS

Crystal structure of Hook3(553-624)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	93
Detector technology	CCD
Collection date	2021-10-25
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.987
Spacegroup name	P 65 2 2
Unit cell lengths	78.747, 78.747, 180.036
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	36.074 - 2.699
R-factor	0.2334
Rwork	0.230
R-free	0.26760
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.100
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	AutoSol
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.750
High resolution limit [Å]	2.699	2.699
Number of reflections	9756	463
<I/σ(I)>	26.5
Completeness [%]	99.0
Redundancy	9.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.1 M Bis-Tris propane (pH 6.8) and 60% Tacsimate (pH 7.0)