9KNJ
Crystal structure of glycerol-bound full-length PHA synthase (PhaC) from Aeromonas caviae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-07-12 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.999994 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 157.032, 96.071, 143.296 |
| Unit cell angles | 90.00, 90.42, 90.00 |
Refinement procedure
| Resolution | 48.040 - 2.700 |
| R-factor | 0.2115 |
| Rwork | 0.210 |
| R-free | 0.24870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.761 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.840 |
| High resolution limit [Å] | 2.680 | 2.680 |
| Rmerge | 0.058 | 0.574 |
| Rmeas | 0.069 | 0.677 |
| Rpim | 0.036 | |
| Number of reflections | 59959 | 9640 |
| <I/σ(I)> | 14.83 | |
| Completeness [%] | 99.8 | 99.7 |
| Redundancy | 3.5 | 3.6 |
| CC(1/2) | 0.999 | 0.816 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277.15 | Sodium chloride, glycerol, PEG6000, octyl beta-D-glucopyranoside |
