Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-04-21 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9735 |
| Spacegroup name | H 3 |
| Unit cell lengths | 141.410, 141.410, 83.850 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 31.480 - 2.630 |
| R-factor | 0.2 |
| Rwork | 0.199 |
| R-free | 0.22730 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.574 |
| Data reduction software | HKL-3000 |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.19.1_4122: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.450 | 2.700 |
| High resolution limit [Å] | 2.630 | 2.630 |
| Rmerge | 0.067 | |
| Rmeas | 0.070 | |
| Number of reflections | 18535 | 18522 |
| <I/σ(I)> | 12.2 | |
| Completeness [%] | 99.7 | |
| Redundancy | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 100 mM sodium citrate, pH 5.4, 12.0% (w/v) polyethylene glycol (PEG) 3350, 8% isopropanol |
