9KN8
Crystal structure of Horse spleen L-ferritin mutant (E53F/E56F/E57F/R59F/E60F/E63F) with Coumarin 153
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E DW |
| Temperature [K] | 93 |
| Detector technology | PIXEL |
| Collection date | 2021-03-08 |
| Detector | RIGAKU HyPix-6000HE |
| Wavelength(s) | 1.54184 |
| Spacegroup name | F 4 3 2 |
| Unit cell lengths | 181.158, 181.158, 181.158 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 18.210 - 1.640 |
| R-factor | 0.1933 |
| Rwork | 0.193 |
| R-free | 0.20820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dat |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.729 |
| Data reduction software | CrysalisPro |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 18.210 | 1.670 |
| High resolution limit [Å] | 1.640 | 1.640 |
| Rmerge | 0.071 | 0.815 |
| Rmeas | 0.075 | 0.903 |
| Rpim | 0.024 | 0.378 |
| Number of reflections | 31536 | 1587 |
| <I/σ(I)> | 20.9 | 2.1 |
| Completeness [%] | 99.5 | 99.4 |
| Redundancy | 8.4 | 5.2 |
| CC(1/2) | 0.999 | 0.819 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | Ammonium sulfate, Cadmium sulfate |
