9KN7

Crystal structure of Horse spleen L-ferritin mutant (Fr-E53F/E56F/E57F/R59L/E60F/E63F)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E DW
Temperature [K]	93
Detector technology	PIXEL
Collection date	2021-06-16
Detector	RIGAKU HyPix-6000HE
Wavelength(s)	1.54184
Spacegroup name	F 4 3 2
Unit cell lengths	180.715, 180.715, 180.715
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	18.940 - 1.500
R-factor	0.1653
Rwork	0.165
R-free	0.17820
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1dat
RMSD bond length	0.013
RMSD bond angle	1.741
Data reduction software	CrysalisPro
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	18.940	1.530
High resolution limit [Å]	1.500	1.500
Rmerge	0.049	0.396
Rmeas	0.051	0.429
Rpim	0.015	0.165
Number of reflections	40895	1981
<I/σ(I)>	31.8	4.5
Completeness [%]	99.9	100
Redundancy	9.9	6.7
CC(1/2)	1.000	0.941

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	Ammonium sulfate, Cadmium sulfate