9KMA

Crystal structure of the CCA-adding enzyme from Arabidopsis thaliana

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2017-06-09
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	61.680, 63.906, 152.523
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.380 - 3.229
R-factor	0.2605
Rwork	0.259
R-free	0.29390
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.136
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX ((1.16_3549: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.310
High resolution limit [Å]	3.200	6.890	3.200
Rmerge		0.077	0.919
Rmeas		0.088	1.034
Rpim		0.042	0.462
Number of reflections	10054	1069	979
<I/σ(I)>	8.1
Completeness [%]	99.4	95.8	100
Redundancy	4.4	4	4.6
CC(1/2)	0.758	0.989	0.758

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	0.1 M Bis-Tris pH 5.5, 25% (w/v) PEG 3350