9KLU
Crystal structure of a Streptococcal protein G B1 mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-02-25 |
| Detector | DECTRIS PILATUS3 S 2M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 43.506, 75.058, 96.925 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.800 - 2.910 |
| R-factor | 0.2358 |
| Rwork | 0.234 |
| R-free | 0.26610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.058 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.010 |
| High resolution limit [Å] | 2.910 | 2.910 |
| Rmerge | 0.065 | 0.408 |
| Number of reflections | 7301 | 718 |
| <I/σ(I)> | 19.1 | |
| Completeness [%] | 98.8 | |
| Redundancy | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293.15 | Protein concentration: 13.2 mg/mL Precipitant: 1.6 M ammonium sulfate Buffer: 0.1 M MES monohydrate |
