9KLE

Crystal structure of Horse spleen L-ferritin mutant (R52F/E56F/R59F/E63F) with Nile Red

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E DW
Temperature [K]	93
Detector technology	PIXEL
Collection date	2021-03-16
Detector	RIGAKU HyPix-6000HE
Wavelength(s)	1.54184
Spacegroup name	F 4 3 2
Unit cell lengths	181.552, 181.552, 181.552
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.030 - 1.650
R-factor	0.1918
Rwork	0.191
R-free	0.20940
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1dat
RMSD bond length	0.013
RMSD bond angle	1.784
Data reduction software	CrysalisPro
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.030	1.680
High resolution limit [Å]	1.650	1.650
Rmerge	0.077	0.847
Rmeas	0.081	0.920
Rpim	0.025	0.356
Number of reflections	31335	1486
<I/σ(I)>	22.1	2.1
Completeness [%]	99.9	100
Redundancy	10.1	6.6
CC(1/2)	0.999	0.823

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	Ammonium sulfate, Cadmium sulfate