9KL6

Crystal structure of NADP-specific glutamate dehydrogenase Gdh1 from Schizosaccharomyces pombe in complex with alpha-iminoglutarate and NADP+

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	95
Detector technology	PIXEL
Collection date	2019-11-16
Detector	DECTRIS PILATUS3 S 2M
Wavelength(s)	1
Spacegroup name	P 63 2 2
Unit cell lengths	96.965, 96.965, 188.258
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.990 - 1.450
R-factor	0.18095
Rwork	0.179
R-free	0.20967
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5gud
RMSD bond length	0.010
RMSD bond angle	1.782
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.990	1.470
High resolution limit [Å]	1.450	1.450
Rmerge	0.076	0.931
Rmeas	0.078	0.962
Rpim	0.018	0.241
Total number of observations		69598
Number of reflections	92864	4450
<I/σ(I)>	21.5	3.2
Completeness [%]	99.9
Redundancy	19	15.6
CC(1/2)	0.999	0.884

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	0.1M HEPES-NaOH, pH 7.5, 1.6M ammonium sulfate