9KJA

Anti-CRISPR protein AcrVA3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	125
Detector technology	PIXEL
Collection date	2023-12-11
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	44.900, 44.900, 47.340
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.370 - 1.730
R-factor	0.2248
Rwork	0.224
R-free	0.23490
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	AlphaFold
RMSD bond length	0.003
RMSD bond angle	0.543
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.17_3644: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.370	1.793
High resolution limit [Å]	1.730	1.732
Number of reflections	10411	10411
<I/σ(I)>	23.65
Completeness [%]	99.8
Redundancy	12.3
CC(1/2)	1.000	0.810

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	20%(w/v) PEG 8,000, 0.1 M sodium HEPES pH 7.2