9KFY

Crystal structure of synthetic PPR-DYW in its RNA-free state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL45XU
Synchrotron site	SPring-8
Beamline	BL45XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-25
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	83.290, 94.540, 101.560
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.270 - 2.260
R-factor	0.2093
Rwork	0.207
R-free	0.24610
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.573
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.390
High resolution limit [Å]	2.260	2.260
Rmerge	0.123
Rmeas	0.123	2.569
Number of reflections	38124	5252
<I/σ(I)>	20.7
Completeness [%]	99.6
Redundancy	18.2
CC(1/2)	0.997	0.981

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	95mM Sodium citrate tribasic dihydrate pH 5.6, 19% 2-Propanol, 5% Glycerol