9KFX

Crystal structure of synthetic PPR-DYW in complex with target RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL45XU
Synchrotron site	SPring-8
Beamline	BL45XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-01-16
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	103.660, 109.350, 187.410
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.360 - 2.700
R-factor	0.2004
Rwork	0.199
R-free	0.24330
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.518
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.850
High resolution limit [Å]	2.700	2.700
Rmeas	0.208	2.967
Number of reflections	59240	8353
<I/σ(I)>	13.8	1.21
Completeness [%]	100.0
Redundancy	17
CC(1/2)	0.998	0.564

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	1000mM Sodium citrate tribasic, 100mM Tris-HCl pH 7.0, 200mM NaCl