9KFO

Structure of WDR5 in complex with mutated EMBOW

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-10-26
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9792
Spacegroup name	P 63
Unit cell lengths	124.350, 124.350, 39.559
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	35.900 - 2.200
R-factor	0.1577
Rwork	0.156
R-free	0.18970
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.079
Data reduction software	autoPROC
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.560	2.270
High resolution limit [Å]	2.200	2.200
Rmerge	0.153	0.730
Rmeas	0.160	0.766
Rpim	0.048	0.230
Total number of observations	196747	16983
Number of reflections	18087	1550
<I/σ(I)>	14.4	5.8
Completeness [%]	100.0
Redundancy	10.9	11
CC(1/2)	0.996	0.920

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M sodium HEPES, pH 7.0, 15 % (w/v) PEG 4000.