9KFN

Structure of WDR5 in complex with mutated EMBOW T3V

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-10-26
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9792
Spacegroup name	P 63
Unit cell lengths	126.112, 126.112, 38.962
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	31.530 - 2.800
R-factor	0.1959
Rwork	0.193
R-free	0.24120
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.794
Data reduction software	PROCOR
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.960	2.950
High resolution limit [Å]	2.800	2.800
Rmerge	0.142	0.904
Rmeas	0.147	0.932
Rpim	0.037	0.222
Total number of observations	142399	22519
Number of reflections	8642	1294
<I/σ(I)>	16.3	5.4
Completeness [%]	96.1
Redundancy	16.5	17.4
CC(1/2)	0.986	0.962

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M imidazole malate, pH 8.5, 12.5 % (w/v) PEG 10000.