9KFF
Truncated Fzo1 with modified LB, transition-like state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL02U1 |
| Synchrotron site | SSRF |
| Beamline | BL02U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-08-21 |
| Detector | DECTRIS EIGER2 S 9M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 77.115, 77.115, 283.551 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.610 - 2.120 |
| R-factor | 0.2043 |
| Rwork | 0.201 |
| R-free | 0.24300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.149 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19_4092) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.610 | 2.180 |
| High resolution limit [Å] | 2.120 | 2.120 |
| Number of reflections | 28505 | 2342 |
| <I/σ(I)> | 9.8 | 3.3 |
| Completeness [%] | 97.3 | 99.4 |
| Redundancy | 6.4 | 6.4 |
| CC(1/2) | 0.974 | 0.697 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277.15 | 0.1M MES(PH 6.5), 8%(w/v) PEG6000 |
