9KFD

Truncated Fzo1,GTP-bound

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2018-05-29
Detector	RAYONIX MX-225
Wavelength(s)	0.97935
Spacegroup name	I 41
Unit cell lengths	174.404, 174.404, 127.001
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	66.460 - 2.730
R-factor	0.2344
Rwork	0.233
R-free	0.26280
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.625
Data reduction software	autoPROC
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19-4092)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	123.320	2.870
High resolution limit [Å]	2.730	2.730
Number of reflections	49576	7355
<I/σ(I)>	5.9	2.3
Completeness [%]	97.8	99.8
Redundancy	13.8	13.3
CC(1/2)	0.978	0.904

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277.15	0.1 M Sodium chloride 0.1 M BIS-TRIS,pH 6.5 1.5 M Ammonium sulfate