9KFB
RABV-G-ecto/NM57-scFv complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-12-21 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97925 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 157.619, 157.619, 221.392 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.640 - 4.300 |
| R-factor | 0.2634 |
| Rwork | 0.261 |
| R-free | 0.31430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.102 |
| Data reduction software | DENZO |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 4.450 |
| High resolution limit [Å] | 4.280 | 4.300 |
| Rmerge | 0.226 | 1.847 |
| Number of reflections | 19823 | 1936 |
| <I/σ(I)> | 24.96 | |
| Completeness [%] | 99.8 | |
| Redundancy | 24.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.2 M Ammonium sulfate, 0.1 M Sodium acetate (pH 5.5), 10 % w/v PEG 2000 MME |
