9KF8
Crystal structure of ferritin mutant (E53H/E57H/E60H/R64H)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E DW |
| Temperature [K] | 93 |
| Detector technology | PIXEL |
| Collection date | 2022-04-25 |
| Detector | RIGAKU HyPix-6000HE |
| Wavelength(s) | 1.543 |
| Spacegroup name | F 4 3 2 |
| Unit cell lengths | 180.930, 180.930, 180.930 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.237 - 1.501 |
| Rwork | 0.156 |
| R-free | 0.17060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.957 |
| Data reduction software | CrysalisPro |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.240 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.072 | 0.608 |
| Rmeas | 0.671 | |
| Rpim | 0.033 | 0.276 |
| Number of reflections | 41042 | 1994 |
| <I/σ(I)> | 21.4 | 2.8 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 8.9 | 5.7 |
| CC(1/2) | 0.999 | 0.819 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | Precipitant: (NH4)2SO4 (0.5-1.0M), CdSO4(12-20mM) Protein: 15-20mg/ml Crystallization drop: 1:1 |
