9KF7
Crystal structure of ferritin mutant (R52H/E56H/R59H/E63H)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E DW |
| Temperature [K] | 93 |
| Detector technology | PIXEL |
| Collection date | 2022-09-27 |
| Detector | RIGAKU HyPix-6000HE |
| Wavelength(s) | 1.543 |
| Spacegroup name | F 4 3 2 |
| Unit cell lengths | 181.700, 181.700, 181.700 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 23.655 - 1.500 |
| Rwork | 0.168 |
| R-free | 0.18680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.759 |
| Data reduction software | CrysalisPro |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.670 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.088 | 0.673 |
| Rmeas | 0.095 | |
| Rpim | 0.468 | |
| Number of reflections | 41552 | 2012 |
| <I/σ(I)> | 18.1 | 2.1 |
| Completeness [%] | 100.0 | 99.6 |
| Redundancy | 7.1 | |
| CC(1/2) | 0.998 | 0.760 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | Precipitant: (NH4)2SO4 (0.5-1.0M), CdSO4(12-20mM) Protein: 15-20mg/ml Crystallization drop: 1:1 |
