9KF5

Crystal structure of Ferritin mutant(E53H/E57H)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E+ DW
Temperature [K]	93
Detector technology	PIXEL
Collection date	2022-11-22
Detector	RIGAKU HyPix-6000HE
Wavelength(s)	1.543
Spacegroup name	F 4 3 2
Unit cell lengths	182.681, 182.681, 182.681
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	23.783 - 1.500
Rwork	0.219
R-free	0.24090
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.707
Data reduction software	CrysalisPro
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	23.810	1.660
High resolution limit [Å]	1.500	1.500
Rmerge	0.102	0.697
Rmeas		0.769
Number of reflections	42036	1599
<I/σ(I)>	16.1	2.1
Completeness [%]	99.4
Redundancy	7.2
CC(1/2)	0.996	0.796

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	Precipitant: (NH4)2SO4 (0.5-1.0M); CdSO4(12-20mM) Protein: 15-20mg/ml Crystallization drop: 1:1