9KF1
Crystal structure of Streptomyces sp. sulfotransferase Cpz8 involved in Caprazamycins Synthesis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL45XU |
| Synchrotron site | SPring-8 |
| Beamline | BL45XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-10-10 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 129.420, 33.040, 64.240 |
| Unit cell angles | 90.00, 102.88, 90.00 |
Refinement procedure
| Resolution | 31.540 - 1.380 |
| R-factor | 0.1421 |
| Rwork | 0.142 |
| R-free | 0.15370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5tiz |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.196 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.1_5286) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.410 |
| High resolution limit [Å] | 1.380 | 1.380 |
| Number of reflections | 54909 | 3690 |
| <I/σ(I)> | 18.1 | |
| Completeness [%] | 99.8 | |
| Redundancy | 7.8 | |
| CC(1/2) | 0.990 | 0.890 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 297 | Sodium phosphate monohydrate monobasic, potassium phosphate dibasic |
