9KEP
RABV-G-PHD-FD/NM57-scFv
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-04-20 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9789 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 114.330, 49.480, 100.860 |
| Unit cell angles | 90.00, 117.62, 90.00 |
Refinement procedure
| Resolution | 29.790 - 2.890 |
| R-factor | 0.2355 |
| Rwork | 0.233 |
| R-free | 0.28110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.814 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.790 | 2.970 |
| High resolution limit [Å] | 2.890 | 2.890 |
| Rmerge | 0.142 | 1.373 |
| Number of reflections | 11423 | 11423 |
| <I/σ(I)> | 11.3 | |
| Completeness [%] | 99.9 | |
| Redundancy | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 180 mM Calcium chloride, 24% PEG3350, 4% v/v polypropylene glycol P 400, and 20% v/v Glycerol |
