9KD4
Structure of WDR5 in complex with WIN motif containing Kif2A 114-120
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-10-26 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 116.867, 47.271, 129.255 |
| Unit cell angles | 90.00, 113.56, 90.00 |
Refinement procedure
| Resolution | 51.190 - 1.640 |
| R-factor | 0.179 |
| Rwork | 0.178 |
| R-free | 0.20400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.295 |
| Data reduction software | PROCOR |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.19.2_4158: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.240 | 1.730 |
| High resolution limit [Å] | 1.640 | 1.640 |
| Rmerge | 0.121 | 0.884 |
| Rmeas | 0.144 | 1.087 |
| Rpim | 0.077 | 0.623 |
| Total number of observations | 263132 | 32682 |
| Number of reflections | 78758 | 11518 |
| <I/σ(I)> | 4.5 | 1.9 |
| Completeness [%] | 98.9 | |
| Redundancy | 3.3 | 2.8 |
| CC(1/2) | 0.976 | 0.491 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M Ammonium acetate, 0.1 M Sodium acetate pH=4, 15% PEG 4000 |
