9KAT
Crystal structure of anti-sulfonylurea antibody scFv apo form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2024-06-29 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.999 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 75.077, 79.659, 85.604 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.160 - 1.510 |
| R-factor | 0.2004 |
| Rwork | 0.200 |
| R-free | 0.21390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.372 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.18.2_3874: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.160 | 1.564 |
| High resolution limit [Å] | 1.510 | 1.510 |
| Rmerge | 0.065 | |
| Rmeas | 0.068 | |
| Rpim | 0.019 | 0.613 |
| Number of reflections | 81110 | 8000 |
| <I/σ(I)> | 19.67 | |
| Completeness [%] | 99.9 | |
| Redundancy | 13.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 0.49 M Sodium phosphate monobasic monohydrate, 0.91 M Potassium phosphate dibasic, pH 6.8 |
