9KA8
Structure of the recombinant structure of the subunit of allophycocyanin (APC) and the formate dehydrogenase (FDH)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 293 |
| Detector technology | PIXEL |
| Collection date | 2024-05-04 |
| Detector | DECTRIS EIGER R 4M |
| Wavelength(s) | 0.987 |
| Spacegroup name | H 3 |
| Unit cell lengths | 189.784, 189.784, 153.879 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.400 - 2.190 |
| R-factor | 0.1887 |
| Rwork | 0.188 |
| R-free | 0.21720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.060 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21_5207) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.400 | 2.250 |
| High resolution limit [Å] | 2.190 | 2.190 |
| Number of reflections | 105397 | 7153 |
| <I/σ(I)> | 3.08 | |
| Completeness [%] | 99.7 | |
| Redundancy | 4.9 | |
| CC(1/2) | 0.974 | 0.974 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 0.1 M NaCl, 0.1M Hepes (pH 7.5), 1.6 M (NH4)2SO4 |
