9K9Z

Room-temperature structure of lysozyme determined by serial synchrotron crystallography (MOSFLM)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 11C
Synchrotron site	PAL/PLS
Beamline	11C
Temperature [K]	298.5
Detector technology	PIXEL
Collection date	2019-02-25
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9794
Spacegroup name	P 43 21 2
Unit cell lengths	79.030, 79.030, 38.240
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.510 - 1.800
R-factor	0.179
Rwork	0.177
R-free	0.20760
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.757
Data reduction software	CrystFEL
Data scaling software	CrystFEL
Phasing software	PHASER
Refinement software	PHENIX ((1.17.1_3660: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	80.000	1.860
High resolution limit [Å]	1.800	1.800
Number of reflections	11708	1121
<I/σ(I)>	6.52
Completeness [%]	100.0
Redundancy	359.7
CC(1/2)	0.984	0.695

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	SMALL TUBES		293.5	Na-acetate, NaCl, PEG 8000