9K9P

crystal structure of Arabidopsis DCL4 dsRBD2 in complex with DRB4 CTD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-12-11
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	32.663, 44.985, 89.412
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.700 - 1.420
R-factor	0.1789
Rwork	0.177
R-free	0.19900
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.972
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.710	1.471
High resolution limit [Å]	1.420	1.420
Number of reflections	25450	2307
<I/σ(I)>	0.941
Completeness [%]	99.1
Redundancy	10.8
CC(1/2)	0.941	0.941

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	0.1 M HEPES,pH 7.5,25% (w/v) PEG1000