9K9M

X-ray structure of FBB18 from Chlamydomonas reinhardtii.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-02-06
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.90000
Spacegroup name	C 2 2 21
Unit cell lengths	122.954, 152.093, 116.602
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.610 - 2.300
R-factor	0.2003
Rwork	0.198
R-free	0.25060
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	0.954
Data reduction software	XDS
Data scaling software	XDS
Phasing software	AutoSol
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.440
High resolution limit [Å]	2.300	2.300
Rmerge	0.054	0.662
Number of reflections	91792	14545
<I/σ(I)>	11.9	1.6
Completeness [%]	98.0
Redundancy	2.8
CC(1/2)	0.998	0.732

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277.15	18% PEG8000, 100 mM HEPES (pH7.5)