9K7R

Crystal structure of DNA/RNA duplex obtained using the counter diffusion method on Earth (K form)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-06-25
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.98
Spacegroup name	P 61
Unit cell lengths	48.472, 48.472, 45.555
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	24.240 - 1.590
R-factor	0.1823
Rwork	0.181
R-free	0.19610
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.043
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	24.240	24.240	1.700
High resolution limit [Å]	1.590	5.000	1.600
Rmerge	0.035	0.023	0.386
Rmeas	0.039	0.026	0.433
Number of reflections	8199	280	1353
<I/σ(I)>	22.36
Completeness [%]	99.9
Redundancy	5.1
CC(1/2)	1.000	0.999	0.924

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	COUNTER-DIFFUSION		293.15	potassium chloride, MPD, spermine, MES