9K7O
Coprinopsis cinerea GH131 protein CcGH131B
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NE3A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-06-24 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 61 |
| Unit cell lengths | 71.849, 71.849, 225.802 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 36.550 - 1.900 |
| R-factor | 0.18164 |
| Rwork | 0.180 |
| R-free | 0.21575 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.391 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0415) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.900 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.119 | 0.457 |
| Rpim | 0.041 | 0.159 |
| Number of reflections | 51549 | 5122 |
| <I/σ(I)> | 28.6 | 4 |
| Completeness [%] | 100.0 | |
| Redundancy | 9.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | PEG 10000, PEG 20000, MES-NaOH |
