9K7L
Gamma-glutamyl peptidase 1 from Arabidopsis thaliana (H192N gamma-Glu intermediate)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NE3A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-05-20 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 71.508, 75.109, 101.206 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.150 - 1.590 |
| R-factor | 0.16816 |
| Rwork | 0.166 |
| R-free | 0.20036 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.843 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.150 | 1.620 |
| High resolution limit [Å] | 1.590 | 1.590 |
| Rmerge | 0.060 | 0.603 |
| Rpim | 0.025 | 0.279 |
| Number of reflections | 71347 | 2936 |
| <I/σ(I)> | 16.2 | 1.8 |
| Completeness [%] | 97.0 | 82.7 |
| Redundancy | 6.6 | 5.4 |
| CC(1/2) | 0.996 | 0.791 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 277 | sodium acetate, sodium dihydrogen phsophate, di-potassium hydrogen phsophate |
