9K7K
Gamma-glutamyl peptidase 1 from Arabidopsis thaliana (inactive form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-09-16 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 72.354, 75.329, 102.583 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.550 - 2.040 |
| R-factor | 0.17955 |
| Rwork | 0.177 |
| R-free | 0.23323 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 2.554 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.550 | 2.100 |
| High resolution limit [Å] | 2.040 | 2.040 |
| Rmerge | 0.096 | 0.569 |
| Rpim | 0.040 | 0.232 |
| Number of reflections | 36242 | 2787 |
| <I/σ(I)> | 13.5 | 3.4 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 6.7 | 6.9 |
| CC(1/2) | 0.998 | 0.895 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 277 | sodium acetate, sodium dihydrogen phsophate, di-potassium hydrogen phsophate |
