9K7I
Gamma-glutamyl peptidase 1 from Arabidopsis thaliana (Glu complex)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL45XU |
| Synchrotron site | SPring-8 |
| Beamline | BL45XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-01-31 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 72.289, 75.816, 102.772 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.670 - 1.740 |
| R-factor | 0.15708 |
| Rwork | 0.155 |
| R-free | 0.19525 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 2.340 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.670 | 1.780 |
| High resolution limit [Å] | 1.740 | 1.740 |
| Rmerge | 0.038 | 0.405 |
| Rpim | 0.038 | 0.405 |
| Number of reflections | 58231 | 3148 |
| <I/σ(I)> | 15.3 | 1.8 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 1.6 | 1.9 |
| CC(1/2) | 0.999 | 0.645 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4 | 277 | sodium acetate, sodium dihydrogen phsophate, di-potassium hydrogen phsophate, glutamate |
