9K3W
Crystal structure of putative transcription regulator (dr_2454) from Deinococcus radiodurans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | RRCAT INDUS-2 BEAMLINE PX-BL21 |
| Synchrotron site | RRCAT INDUS-2 |
| Beamline | PX-BL21 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2022-06-20 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.977 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 48.779, 29.618, 115.750 |
| Unit cell angles | 90.00, 93.36, 90.00 |
Refinement procedure
| Resolution | 25.300 - 1.600 |
| R-factor | 0.2083 |
| Rwork | 0.206 |
| R-free | 0.24570 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.823 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21_5207) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.300 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.023 | 0.947 |
| Number of reflections | 21529 | 1051 |
| <I/σ(I)> | 22.6 | 2.2 |
| Completeness [%] | 97.4 | 95.6 |
| Redundancy | 7.5 | 7.6 |
| CC(1/2) | 0.999 | 0.819 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 294 | 0.1M HEPES pH7 30% Jeffamine ED-2001 pH7 |
