9K2U

Crystal structure of HLA-C*1202 in complex with IY11V9A peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-24
Detector	DECTRIS EIGER X 4M
Wavelength(s)	1.10000
Spacegroup name	I 2 2 2
Unit cell lengths	78.138, 100.555, 113.744
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.820 - 2.100
R-factor	0.2201
Rwork	0.219
R-free	0.24710
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.646
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX (1.21_5207)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.820	2.160
High resolution limit [Å]	2.100	2.100
Number of reflections	26522	2132
<I/σ(I)>	12.7
Completeness [%]	100.0
Redundancy	13.7
CC(1/2)	0.997	0.866

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2M Sodium bromide, 20% (w/v) PEG 3350