9K2S

Killer cell immunoglobulin-like receptor 2DL2 in complex with HLA-C*1202 and IY10 peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-19
Detector	DECTRIS EIGER X 4M
Wavelength(s)	1.10000
Spacegroup name	P 1 21 1
Unit cell lengths	84.493, 44.585, 88.243
Unit cell angles	90.00, 111.45, 90.00

Refinement procedure

Resolution	48.610 - 2.400
R-factor	0.2474
Rwork	0.245
R-free	0.29940
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.151
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.610	2.490
High resolution limit [Å]	2.400	2.400
Number of reflections	22791	2274
<I/σ(I)>	10.1
Completeness [%]	93.7
Redundancy	10.1
CC(1/2)	0.985	0.886

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2M K/Na tartrate, 20% PEG 3350