9K2N

Crystal structure of Glutamine Synthetase-apo

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 11.2C
Synchrotron site	ELETTRA
Beamline	11.2C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-04-24
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0
Spacegroup name	P 43 21 2
Unit cell lengths	260.909, 260.909, 154.180
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	93.215 - 3.400
Rwork	0.233
R-free	0.27020
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	2.160
Data reduction software	iMOSFLM (ccp4 9.0.001)
Data scaling software	Aimless (ccp4 9.0.001)
Phasing software	MOLREP (ccp4 9.0.001)
Refinement software	REFMAC (5.8.0430 (refmacat 0.4.100))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	99.590	3.470
High resolution limit [Å]	3.400	3.400
Number of reflections	72108	4374
<I/σ(I)>	9	2.6
Completeness [%]	98.5	98.1
Redundancy	6.8	7.1
CC(1/2)	0.967	0.360

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	293	0.1M NaCl, 0.1M BICINE pH 9.0, 5mM MgCl2, 20% v/v polyethylene glycol monomethyl ether 550